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quantitative fluorescence intensity analysis of tlr4 protein expression  (MetaMorph Inc)

 
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    MetaMorph Inc quantitative fluorescence intensity analysis of tlr4 protein expression
    List of the antibodies used in Western blot analysis
    Quantitative Fluorescence Intensity Analysis Of Tlr4 Protein Expression, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative fluorescence intensity analysis of tlr4 protein expression/product/MetaMorph Inc
    Average 90 stars, based on 1 article reviews
    quantitative fluorescence intensity analysis of tlr4 protein expression - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice"

    Article Title: Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice

    Journal: American Journal of Physiology - Renal Physiology

    doi: 10.1152/ajprenal.00621.2018

    List of the antibodies used in Western blot analysis
    Figure Legend Snippet: List of the antibodies used in Western blot analysis

    Techniques Used: Western Blot

    Forward and reverse sequences of primers that were used to amplify specific mRNAs in the kidney tissue of natriuretic peptide receptor-A gene-targeted mice
    Figure Legend Snippet: Forward and reverse sequences of primers that were used to amplify specific mRNAs in the kidney tissue of natriuretic peptide receptor-A gene-targeted mice

    Techniques Used: Sequencing

    Analysis of Toll-like receptors (TLRs) and mammalian target of rapamycin (mTOR) expression in natriuretic peptide receptor-A (Npr1) gene-targeted kidneys in untreated mice and mice treated with rapamycin. A–C: relative mRNA expression of TLR2-, TLR4-, and TLR6-targeted genes normalized to β-actin mRNA in kidney tissues. D: renal expression levels of TLR4 protein were determined by Western blot analysis. E: immunofluorescence of TLR4 in rapamycin-treated and vehicle-treated Npr1 gene-targeted mouse kidneys. A marked increase in TLR4 expression occurred in Npr1 gene knockout kidneys but not in the kidneys of wild-type controls. Rapamycin decreased expression of TLR4 in tubular epithelial cells of kidneys. Red fluorescence indicates TLR4 protein expression; blue fluorescence indicates a nucleus stained with DAPI. F: quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software. G: mTOR protein expression was determined by Western blot analysis. β-Actin was used as an internal control for normalization. Densitometric analyses of the respective bands were done with an Alpha Innotech phosphoimager. Values are expressed as means ± SE; n = 6/group. *P < 0.05, control and Npr1++/++ vs. rapamycin-treated Npr1++/++ mice; **P < 0.01, control Npr1+/+ and Npr1++/++ vs. rapamycin-treated Npr1+/+ and Npr1++/++ mice; ***P < 0.001, control Npr1+/− and Npr1−/− vs. rapamycin-treated Npr1+/−and Npr1−/− mice.
    Figure Legend Snippet: Analysis of Toll-like receptors (TLRs) and mammalian target of rapamycin (mTOR) expression in natriuretic peptide receptor-A (Npr1) gene-targeted kidneys in untreated mice and mice treated with rapamycin. A–C: relative mRNA expression of TLR2-, TLR4-, and TLR6-targeted genes normalized to β-actin mRNA in kidney tissues. D: renal expression levels of TLR4 protein were determined by Western blot analysis. E: immunofluorescence of TLR4 in rapamycin-treated and vehicle-treated Npr1 gene-targeted mouse kidneys. A marked increase in TLR4 expression occurred in Npr1 gene knockout kidneys but not in the kidneys of wild-type controls. Rapamycin decreased expression of TLR4 in tubular epithelial cells of kidneys. Red fluorescence indicates TLR4 protein expression; blue fluorescence indicates a nucleus stained with DAPI. F: quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software. G: mTOR protein expression was determined by Western blot analysis. β-Actin was used as an internal control for normalization. Densitometric analyses of the respective bands were done with an Alpha Innotech phosphoimager. Values are expressed as means ± SE; n = 6/group. *P < 0.05, control and Npr1++/++ vs. rapamycin-treated Npr1++/++ mice; **P < 0.01, control Npr1+/+ and Npr1++/++ vs. rapamycin-treated Npr1+/+ and Npr1++/++ mice; ***P < 0.001, control Npr1+/− and Npr1−/− vs. rapamycin-treated Npr1+/−and Npr1−/− mice.

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Gene Knockout, Fluorescence, Staining, Software, Control



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    quantitative fluorescence intensity analysis of tlr4 protein expression  (MetaMorph Inc)
    90
    MetaMorph Inc quantitative fluorescence intensity analysis of tlr4 protein expression
    List of the antibodies used in Western blot analysis
    Quantitative Fluorescence Intensity Analysis Of Tlr4 Protein Expression, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative fluorescence intensity analysis of tlr4 protein expression/product/MetaMorph Inc
    Average 90 stars, based on 1 article reviews
    quantitative fluorescence intensity analysis of tlr4 protein expression - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    List of the antibodies used in Western blot analysis

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice

    doi: 10.1152/ajprenal.00621.2018

    Figure Lengend Snippet: List of the antibodies used in Western blot analysis

    Article Snippet: F : quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software.

    Techniques: Western Blot

    Forward and reverse sequences of primers that were used to amplify specific mRNAs in the kidney tissue of natriuretic peptide receptor-A gene-targeted mice

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice

    doi: 10.1152/ajprenal.00621.2018

    Figure Lengend Snippet: Forward and reverse sequences of primers that were used to amplify specific mRNAs in the kidney tissue of natriuretic peptide receptor-A gene-targeted mice

    Article Snippet: F : quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software.

    Techniques: Sequencing

    Analysis of Toll-like receptors (TLRs) and mammalian target of rapamycin (mTOR) expression in natriuretic peptide receptor-A (Npr1) gene-targeted kidneys in untreated mice and mice treated with rapamycin. A–C: relative mRNA expression of TLR2-, TLR4-, and TLR6-targeted genes normalized to β-actin mRNA in kidney tissues. D: renal expression levels of TLR4 protein were determined by Western blot analysis. E: immunofluorescence of TLR4 in rapamycin-treated and vehicle-treated Npr1 gene-targeted mouse kidneys. A marked increase in TLR4 expression occurred in Npr1 gene knockout kidneys but not in the kidneys of wild-type controls. Rapamycin decreased expression of TLR4 in tubular epithelial cells of kidneys. Red fluorescence indicates TLR4 protein expression; blue fluorescence indicates a nucleus stained with DAPI. F: quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software. G: mTOR protein expression was determined by Western blot analysis. β-Actin was used as an internal control for normalization. Densitometric analyses of the respective bands were done with an Alpha Innotech phosphoimager. Values are expressed as means ± SE; n = 6/group. *P < 0.05, control and Npr1++/++ vs. rapamycin-treated Npr1++/++ mice; **P < 0.01, control Npr1+/+ and Npr1++/++ vs. rapamycin-treated Npr1+/+ and Npr1++/++ mice; ***P < 0.001, control Npr1+/− and Npr1−/− vs. rapamycin-treated Npr1+/−and Npr1−/− mice.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Genetic disruption of Npr1 depletes regulatory T cells and provokes high levels of proinflammatory cytokines and fibrosis in the kidneys of female mutant mice

    doi: 10.1152/ajprenal.00621.2018

    Figure Lengend Snippet: Analysis of Toll-like receptors (TLRs) and mammalian target of rapamycin (mTOR) expression in natriuretic peptide receptor-A (Npr1) gene-targeted kidneys in untreated mice and mice treated with rapamycin. A–C: relative mRNA expression of TLR2-, TLR4-, and TLR6-targeted genes normalized to β-actin mRNA in kidney tissues. D: renal expression levels of TLR4 protein were determined by Western blot analysis. E: immunofluorescence of TLR4 in rapamycin-treated and vehicle-treated Npr1 gene-targeted mouse kidneys. A marked increase in TLR4 expression occurred in Npr1 gene knockout kidneys but not in the kidneys of wild-type controls. Rapamycin decreased expression of TLR4 in tubular epithelial cells of kidneys. Red fluorescence indicates TLR4 protein expression; blue fluorescence indicates a nucleus stained with DAPI. F: quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software. G: mTOR protein expression was determined by Western blot analysis. β-Actin was used as an internal control for normalization. Densitometric analyses of the respective bands were done with an Alpha Innotech phosphoimager. Values are expressed as means ± SE; n = 6/group. *P < 0.05, control and Npr1++/++ vs. rapamycin-treated Npr1++/++ mice; **P < 0.01, control Npr1+/+ and Npr1++/++ vs. rapamycin-treated Npr1+/+ and Npr1++/++ mice; ***P < 0.001, control Npr1+/− and Npr1−/− vs. rapamycin-treated Npr1+/−and Npr1−/− mice.

    Article Snippet: F : quantitative fluorescence intensity analysis of TLR4 protein expression was done by measuring intracellular fluorescence using MetaMorph software.

    Techniques: Expressing, Western Blot, Immunofluorescence, Gene Knockout, Fluorescence, Staining, Software, Control